Table 4.
Agonista | Antagonistb | |
---|---|---|
Average signal-to-basal ratio | 2.51 ± 0.40 | 2.14 ± 0.36 |
Average Z′ factor | 0.71 ± 0.11 | 0.59 ± 0.13 |
Average %CV | 0.8 ± 0.4 | 7.3 ± 2.5 |
The means of averaged replicate values from 6 different assays were used for statistical calculations. Error reported as standard deviation.
For the agonist mode (0–220 s), signal and basal values were defined as the maximum response fluorescence resulting from EC100 and EC0 concentrations of NPS challenge, respectively.
For the antagonist mode (221–360 s), signal and basal values were defined as the maximum response fluorescence resulting from IC0 and IC100 concentrations of a NPSR antagonist in the presence of an EC90 challenge of NPS.