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. 2010 Jun;8(3):367–379. doi: 10.1089/adt.2009.0245

Table 4.

Statistical Parameters of the DMSO Plate

  Agonista Antagonistb
Average signal-to-basal ratio 2.51 ± 0.40 2.14 ± 0.36
Average Z′ factor 0.71 ± 0.11 0.59 ± 0.13
Average %CV 0.8 ± 0.4 7.3 ± 2.5

The means of averaged replicate values from 6 different assays were used for statistical calculations. Error reported as standard deviation.

a

For the agonist mode (0–220 s), signal and basal values were defined as the maximum response fluorescence resulting from EC100 and EC0 concentrations of NPS challenge, respectively.

b

For the antagonist mode (221–360 s), signal and basal values were defined as the maximum response fluorescence resulting from IC0 and IC100 concentrations of a NPSR antagonist in the presence of an EC90 challenge of NPS.