Table 5.

Statistical Parameters of the Multiplex GPCR Screening for Agonists and Antagonists of NPSR

	First Run		Second Run
	Agonist	Antagonist	Agonist	Antagonist
Plates screened	29		29
Total wells	44,544		44,544
Compounds tested	37,888		37,888
Compound final concentration	8.25 μM	6.25 μM	8.25 μM	6.25 μM
Average Z′ factor	0.81 ± 0.04	0.78 ± 0.09	0.76 ± 0.08	0.73 ± 0.19
%CV	2.5 ± 1.7	6.8 ± 2.2	2.2 ± 1.9	6.0 ± 1.9
Hit cutoff	60% activation	60% inhibition	60% activation	60% inhibition
Hit number	46	124	37	172
Hit rate	0.1%	0.3%	0.1%	0.4%