Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Apr 11;1799(7):502–509. doi: 10.1016/j.bbagrm.2010.04.001

Figure 3. PKD1-BS1 mediates heterologous transcriptional repression.

Figure 3

A. A schematic representation of control and mutant rat bradykinin B2 receptor (Bdkrb2) promoter reporter constructs. The Bdkrb2 promoter has two p53-consensus sequences, P1 (enhancer) and P2 (repressor). B. Bdkrb2-CAT constructs P2/P1, P2/ΔP1 or P2/BS1 were transfected into IMCD3 cells along with the indicated amount of pCMV-p53. C. BdkrB2-CAT constructs P2/P1, ΔP2/P1 or ΔP2/BS1 were transfected into IMCD3 cells along with the indicated amount of pCMV-p53. Cell lysates were harvested 24 h post-transfection and assayed for chloramphenicol acetyltransferase activity. Data represent mean ± S.E. of at least three experiments, * p<0.05; # p<0.001.