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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Apr 11;1799(7):502–509. doi: 10.1016/j.bbagrm.2010.04.001

Figure 5. Effect of Trichostatin A (TSA), an HDAC inhibitor, on PKD1 promoter activity.

Figure 5

A. IMCD-3 cells were treated with TSA (50 ng/ml) or vehicle 2hrs before transfection with PKD1 luciferase constructs. pSV-LacZ was co-transfected to monitor transfection efficiency. Cell lysates were harvested 24 h post-transfection and assayed for luciferase activity. Data represent mean ± S.E. of at least three experiments, *p<0.05. B. Western blot analysis demonstrating the presence of p53, mSin3A, and HDAC1/2 in IMCD3 cells. C. Chromatin immunoprecipitation analysis. PKD1-Luc promoter construct was transfected into IMCD3 cells. Twenty hour later, transfected cells were subjected to ChIP-PCR analysis as detailed in the Methods section using human PKD1-specific primers (arrowheads).