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Sequence of mutagenesis primers used to generate pBdkrB2 P2/BS1 and pBdkrB2 - ΔP2/BS1 constructs.
hPKD1 p53BS1 sequence: 5′-GGTCGCGCTGTGGCGAAGGGGGCGGAGCCTGCAC-3′
Forward mutagenesis primers
| 5′-CTGGAAGTGGAGGGGGGTCGCTGACATCACCGGCCAG |
| 5′-GTGGAGGGGGGTCGCGCTGTGTGACATCACCGGCCAG |
| 5′-GGGGGGTCGCGCTGTGGCGAAGTGACATCACCGGCCAG |
| 5′-CGCGCTGTGGCGAAGGGGGCGTGACATCACCGGCCAG |
| 5′-GTGGCGAAGGGGGCGGAGCCTTGACATCACCGGCCAG |
| 5′-CGAAGGGGGCGGAGCCTGCACTGACATCACCGGCCAG |
