Table 2.

Sequence of mutagenesis primers used to generate mutant PKD1-luciferase constructs. The 10bp deletions are indicated in bold.

PKD1 p53BS1 sequence: 5′-GGTCGCGCTGTGGCGAAGGGGGCGGAGCCTGCAC-3′

PKD1 p53BS2 sequence: 5′-CTACAAGCGTGAGCCAGTTT-3′

PKD1 p53BS3 sequence: 5′-GAACTTCTGATCTTGTGATGTGCCC-3′

PKD1 p53BS4 sequence: 5′-AGTCTCACTCTGTCACCCAGGCTGGAGT-3′

Forward Mutagenesis primers

5′-CAGTCCCTCATCGCTGGCCCTGGCGAAGGGGGCGGAGCC	10bp deletion p53BS1
5′-GCCTCTCGAGTACCTGGGAGAGCCAGTTTGGCTATTTTGG	10bp deletion p53BS2
5′-GGCTAGGCTGGTCTCTCTGATCTTGTGATC	1st 5bp deletion p53BS3
5′-CACGTTGGCTAGGCTGGTCTCTCTTGTGATCTGCCCG	2nd 5bp deletion p53BS3
5′-CAGAGTCTCACTCTGTCACCCGAAGTGGCGGGATCTCGGC	1st 5bp deletion p53BS4
5′-CTCACTCTGTCACCCGGAGTGAAGTGGCGG	2nd 5bp deletion p53BS4