Fig. 2.
CXCL10 plays a critical role in CNS glioma homing of Tc1 cells. WT C57BL/6 mice bearing i.c. GL261 tumors received i.m. injections of poly-ICLC (20 μg/dose) or control PBS on days 20 and 23. Mice treated with poly-ICLC received i.p. injections of anti-mouse CXCL10 mAb (250 μg/dose) or the same amounts of hamster IgG on day 20 and 100 µg on days 21, 22, 23, and 24. All mice received a single i.v. infusion of 3 × 106 pmel-1 derived Tc1 cells on day 21. Mice were sacrificed on day 25 and BILs were analyzed by flow cytometry. a Numbers in each dot plot indicate the percentage of CD8+/gp100-tetramer+ T cells in BILs. b Total numbers of CD8+/GP100-tetramer+ T cells in BILs per mouse. Results from one of two experiments with similar results are shown. Due to the small number of BILs obtained per mouse, BILs obtained from all mice in a given group (5 mice/group) were pooled and then evaluated for the relative number/mouse and phenotype of the BILs between groups