Table 4.
Comparison among Hprt mutation spectra in EM-C11 and CHO-9 cell lines
| EM-C11 | |||
|---|---|---|---|
| CHO-9a | |||
| Type of Hprt mutations | Spontaneous | Me-lex | |
| induced | Spontaneous | Me-lex induced | |
| n° (%) | n° (%) | ||
| n° (%) | n° (%) | ||
| Base pair changes | 9 (31%) | 7 | |
| (15%) | 16 (43%) | 10 (23%) | |
| AT-targeted | 3 (10%) | 7 | |
| (15%) | 12 (32%) | 7 (16%) | |
| AT>TA | 1 | 2 | |
| 1 | 2 | ||
| AT>GC | 2 | 2 | |
| 9 | 4 | ||
| AT > CG | 0 | 3 | |
| 2 | 2 | ||
| GC-targeted | 6 (21%) | 0 | |
| 4 (11%) | 3 (7%) | ||
| GC > AT | 0 | 0 | |
| 0 | 1 | ||
| GC > CG | 0 | 0 | |
| 1 | 0 | ||
| GC > TA | 6 | 0 | |
| 3 | 2 | ||
| Splice mutationsb | 3 (10%) | 9 | |
| (19%)c | 1 (3%) | 1(2%)c | |
| Frame shifts | 2 (7%) | 2 | |
| (4.3%) | 0 | 3 (7%) | |
| Genomic deletions | 11 (38%)d | 11 | |
| (24%) | 3 (8%)d | 21 (48%) | |
| Exon deletions | 6 (21%) | 6 | |
| (13%) | 3 | 9 (21%) | |
| Complete Hprt deletions | 5 (17%) | 5 | |
| (11%) | 0 | 12 (27%) | |
| Mutations in regulatory regions | 3 (10.5%)e | 16 | |
| (35%)e | 12 (32%) | 8 (18%) | |
| Other | 1 (3.5%) | 1 | |
| (2%) | 5 (14%) | 1 (2%) | |
| Total n° of mutations | 29 (100%) | 46 | |
| (100%) | 37 (100%) | 44 (100%) | |
a
From Russo et al., 2009 and data unpublished
b
This category includes splice mutants for which the mutation at splice junction was found
c
p=0.0153 (Fisher’s exact test)
d
p=0.0054 (Fisher’s exact test)
e
p=0.0277 (Fisher’s exact test)