Fig. 2.

(a) DNA damage in sperm from one donor exposed to 0, 5, 10 or 25 μM B[a]P (in 1% DMSO) ± human S9-mix. Comet analysis with or without FPG. Data represent the mean % Tail DNA ± 1SE, of three to six individual experiments, and were assessed by one-way analysis of variance (ANOVA) with a Tukeys's post-hoc test. No significant differences were observed for the various treatments (except for the positive control, X-rays 200 Gy; P < 0.05) compared to baseline and vehicle control samples. (b) DNA damage in sperm exposed to 0, 5, 20 or 50 μM BPDE in 1% DMSO. Comet analysis with or without FPG. Data are represented as mean % Tail DNA from four to six individual experiments ± 1SE. Assessed by one-way ANOVA with a Tukeys's post-hoc test. Only exposure to 20 or 50 μM BPDE combined with FPG treatment induced significantly (*P < 0.05) higher DNA damage levels compared to control samples. Positive control: X-rays 200 Gy. Comet assay Protocol 2.