Table II.

Mean values for staining intensity levels in sperm cells of non-smokers and smokers

	STI score	STI 0	STI 1	STI 2	STI 3
Non-smokers, vehicle only (n = 3, mean ± SE)	69.0 ± 2.3	36.0 ± 3.74	59.0 ± 4.8	5.0 ± 1.4	0.0 ± 0
Smokers, vehicle only (n = 3, mean ± SE)	182.0 ± 15.2	3.0 ± 1.87	37.0 ± 5.3	35.0 ± 3.0	25.0 ± 10.0
P-value	0.050	0.050	0.046	0.050	0.037
Non-smokers, B[a]P treated (n = 3, mean ± SE)	186.0 ± 5.1	0.0 ± 0	34.0 ± 3.0	46.0 ± 3.9	20.0 ± 4.3
Smokers, B[a]P treated (n = 3, mean ± SE)	216.0 ± 9.4	0.0 ± 0	22.0 ± 3.2	40.0 ± 5.0	38.0 ± 8.3
P-value	0.050	1.000	0.050	0.275	0.127

Staining intensity levels (STI ± SE) and statistical differences (P-values) are shown for sperm samples treated for 1 h with 40 μM B[a]P plus S9 mix, or vehicle alone and analysed with a monoclonal antibody to detect DNA adducts.