Fig. 3.

Epsilon PKC activation is required for p65 translocation after IPC: a inhibition of epsilon PKC-reduced PC-induced translocation of p65 to nucleus. Cells were treated with epsilon PKC inhibitor (εPKCi was εV1-2, 100 nM) or vehicle (tat carrier peptide) during IPC and reperfusion. Cells were isolated at 1 h of reperfusion after IPC (1 h of OGD). Western blotting for p65 was carried out and the membrane was re-probed with lamin B antibody. b Histogram depicted densitometric analysis of Western blots of p65 in nuclear protein extracts compared with lamin B, respectively. *p<0.05 compared with control, #p<0.05 compared with IPC