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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: Science. 2010 May 28;328(5982):1172–1176. doi: 10.1126/science.1187532

Fig. 1. 4E-BPs mediate mTORC1’s effects on cell proliferation.

Fig. 1

(A) Levels of the indicated proteins in wild-type and 4E-BP DKO MEFs infected with a scrambled or raptor-specific shRNA (sh-raptor) were determined by Western blotting. β actin-loading control. (B) Proliferation of MEFs from (A) was determined by 5-bromo2′-deoxyuridine (BrdU) incorporation. Results represent means ± SD (n=4). (C) Western blots of HEK293T cells infected by raptor and 4E-BP1 and 2 shRNA (sh-raptor; sh-4E-BP1/2), or scrambled shRNA. eIF4E served as a loading control. D Proliferation of cells from (C) was determined by BrdU incorporation. Results represent percentage of the values obtained in dimethyl sulfoxide (DMSO)-treated cells (set to 100%) ± SD values (n=4).