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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: J Muscle Res Cell Motil. 2009 Apr 21;30(1-2):67–72. doi: 10.1007/s10974-009-9176-y

Figure 2. Differential phosphorylation of cTnT and cTnI by PKC-α, βII, δ, ε, PKD and PKA.

Figure 2

Recombinant troponin complexes were subjected to in vitro kinase assays (top) in buffers containing [γ-32P]ATP and either PKCα, PKCβII, PKCδ, PKCε, PKD or PKA. Phosphorylated forms of cTnT and cTnI were separated by SDS-PAGE and visualized by autoradiography. Ponceau staining (bottom) shows equal protein loading in each lane. Results were replicated in three separate experiments.