Figure 4. Inhibition of histone deacetylation mimics signal 3 cytokine effects on CD8 T cell differentiation.

Purified CD44^lo OT-1 CD8 T cells were stimulated for 3 days with Ag-B7 coated aAPC alone or with IL-12, IFNα or trichostatin A (TSA) (7.5ng/ml). TSA was dissolved in DMSO, and controls having Ag-B7 and DMSO were included.

(A-B) Expression of grzB and IFNγ by intracellular staining. Isotype controls were run for all samples (not shown), and gates set so that > 98% of events fell into the lower quadrant for the controls.

(C) Cytolytic activity measured by ⁵¹Cr release assay using E.G7 target cells.