Table II.

Changes in gene expression in response to Ag-B7 alone and with IL-12 or IFNα.¹

		FOLD CHANGE
		$\frac{\mathrm{Ag}\text{-}\mathrm{B}7}{\text{Na}\ddot{\mathrm{i}}\text{ve}\left(0\text{-}\mathrm{h}\right)}$			$\frac{\mathbf{IL}\text{-}12+\mathrm{Ag}\text{-}\mathrm{B}7}{\mathrm{Ag}\text{-}\mathrm{B}7}$			$\frac{\mathrm{IFN}\alpha +\mathrm{Ag}\text{-}\mathrm{B}7}{\mathrm{Ag}\text{-}\mathrm{B}7}$			$\frac{\mathbf{TSA}}{\mathrm{Ag}\text{-}\mathrm{B}7}$
Gene	Accession Number	24h	48h	72h	24h	48h	72h	24h	48h	72h	72h
Cell surface receptor & signal transduction
IL-12Rβ1	U23922	4.6	2.6	*	*	*	2.5	*	*	1.7	1.9
IL-12Rβ2	U64199	3.7	3.8	8.2	1.8	3.7	3.5	*	1.7	2.1	*
IL-7R	M29697	-29.6	-14.6	-1.8	*	2.8	1.7	*	*	*	-3.7
IL-15Rα	U22339	*	*	*	*	*	*	*	1.7	1.8	*
IL-10Rα	L12120	*	*	*	*	*	1.7	*	3.6	4.0	2.1
Tnfsf4 (OX-40L)	U12763	*	*	*	2.6	*	11.1	2.5	*	*	*
Tnfrsf18 (GITR)	U82534	*	2.2	*	*	*	2.8	*	*	1.8	*
Tnfrsf7 (CD27)	L24492	*	*	3.8	*	*	-1.7	*	*	-1.7	-2.8
Gadd45γ	AF055638	*	*	*	3.7	5.4	3.2	*	*	*	5.6
Gadd45β	AV138783	2.5	-2.4	-3.8	*	2.5	2.6	*	4.4	*	2.3
TRAF4	AV109962	*	*	*	*	1.7	2.8	*	*	1.8	2.1
TRAF6	D84655	*	*	*	*	*	1.8	*	*	*	1.9
Cytotoxic function and cytokines (see also Figure 2)
TRAIL / Apo2L	U37522	*	*	*	2.0	2.4	*	*	*	*	*
Granzyme F	J03257	*	*	*		5.2	7.5	*	*	*	*
TNF-α	U16985	-3.3	*	*	*	*	*	*	*	*	9.2
VEGF-A	M95200	5.9	9.2	*	*	2.4	7.5	*	*	5.7	-3.5
Cell adhesion and migration
CD44	X66084	9.5	8.4	8.2	*	*	*	*	*	*	-2.1
CCR7	L31580	-5.9	-3.6	-2.1	*	-2.4	*	*	*	*	-1.9
CXCR3	AF045146	*	2.2	3.9	*	-2.5	-3.5	*	*	-2.1	*
Mac-2/Galectin-3	X16834	21.4	72.5	168	1.9	2.6	3.0	*	*	*	-7.5
L-selectin (CD62L)	M36058	-8.9	-3.2	*	*	*	-3.0	*	*	-2.3	-2.6
CCL9	U49513	2.3	*	*	1.7	*	*	*	*	*	*
CCL3 (MIP-1α)	J04491	12.2	11.8	*	*	*	2.5	*	*	*	8.6
CCL4 (MIP-1β)	X62502	39.0	21.2	1.9	*	*	2.8	*	*	*	8.6
XCL-1	U15607	21.4	12.2	6.4	*	*	1.7	*	*	2.1	5.3
Integrin β7	M68903	-4.9	-2.8	*	*	-2.2	-1.7	*	-1.9	-2.3	-3.7
Galectin-9 ligand	U55060	-2.4	*	*	*	*	-2.0	2.6	2.7	1.8	*
Rho GDI-γ	U73198	6.7	6.1	*	*	*	4.6	*	*	9.2	*
Defense and homeostasis
Glycoprotein 49 A	M65027	-1.5	2.6	*	7.5	12.0	9.2	*	*	*	*
Glycoprotein 49 B	U05265	-3.6	*	*	6.5	16.5	4.6	*	*	*	*
CTLA 2 alpha	X15591	-5.8	*	6.6	2.5	8.5	3.7	*	*	*	*
CTLA 2 beta	X15592	*	*	9.7	*	23.8	3.7	*	*	*	-3.7
Transcription regulation
Bhlhd-B2	Y07836	8.7	14.8	4.8	*	3.0	9.2	*	*	5.6	*
Prdm-1 (BLIMP-1)	U08185	*	*	-1.9	*	3.0	7.5		1.7	2.8	*
Eomesodermin	AW120579	-2.2	-1.9	-2.9	*	*	*	4.0	11.2	4.0	13.9
NF-IL3R	U83148	*	*	*	*	*	6.5	*	*	2.5	*
LKLF 2	U25096	-154	-16.8	-4.1	*	*	19.7	*	*	-3.2	-22.6
LEF-1	D16503	*	*	*	*	*	-2.0	*	*	-2.1	-2.3
Gata binding protein 3	X55123	-3.2	*	1.8	-2.8	-3.9	-4.6	*	*	-2.3	-1.7
TCF-7	AI019193	-4.1	-3.7	*	*	-4.0	-8.0	*	-1.8	-2.6	*
TGFβ inducible egr	AF064088	*	*	3.3	*	-2.9	-17.2	*	*	-4.0	*
POU domain, 2af1	Z54283	*	*	*	*	-2.8	-3.0	*	-1.7	-2.5	*
Idb3	M60523	*	2.2	3.0	*	*	-3.7	*	-2.2	-2.8	*

¹For cells stimulated with Ag and B7, the fold change was calculated by comparing signal intensities with naïve (0 hr) cell signal intensities. For cells stimulated with Ag-B7 in presence of IL-12, IFNα or TSA, the fold change was determined by comparison with cells stimulated with only Ag-B7 at each of the respective time points. IL-2 was present in all cultures. Fold changes are shown only if they were ≥ 1.70 and had a change p-value ≤0.05. An * indicates no change or no significant change based on the selection criteria. Only selected genes are shown: a complete listing of regulated genes is provided in Supplementary Tables. (Note: To avoid duplication the genes shown in figure 2 are not included in Table II).