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Analysis of degradation of Hsp90 client proteins. Protein levels of p53, cyclin D1 (CD1) and estrogen receptor (ER) were determined in cells treated for 3, 6 and 9 hours with 10 μM cisplatin and 10 μM LA-12 by western blotting. CM-1 rabbit polyclonal antibody, SP1 rabbit monoclonal antibody and CD1 mouse monoclonal antibody was used for detection of p53, ER and CD1 proteins, respectively. Actin was used as loading control.
