TABLE 3.

Microbial richness estimation for each DNA sample

DNA sample	Sanger sequencing				% Chao estimate observed (Sanger/ pyrosequencing)b	Pyrosequencing
	No. of sequences	No. of OTUs	Diversity estimate using Chao1	% Chao estimate observeda		No. of sequences	No. of OTUs	Diversity estimate using Chao1	% Chao estimate observedc
1	75	38	88	43.18	2.30	1,761	655	1,654	39.60
2	96	69	289	23.88	3.27	1,818	873	2,107	41.43
3	94	17	45	37.78	0.64	2,338	1,185	2,674	44.32
4	60	64	180	35.56	4.77	1,303	616	1,342	45.90
5	37	36	333.5	10.79	1.38	1,956	992	2,613	37.96
6	74	53	139.7	37.94	3.09	2,120	775	1,714	45.22
7	92	11	39	28.21	2.59	2,109	192	424	45.28
8	89	60	273	21.98	2.26	3,680	1,209	2,659	45.47
9	93	55	213	25.82	1.82	1,898	1,129	3,025	37.32
10	80	58	271	21.40	2.53	2,119	1,056	2,295	46.01
11	85	53	140	37.86	1.28	3,948	1,753	4,149	42.25
12	62	36	148	24.32	1.58	2,705	901	2,280	39.52
13	73	66	361	18.28	2.21	1,459	923	2,991	30.86
14	90	25	55	45.45	1.39	2,375	519	1,795	28.91
15	68	54	178	30.34	2.34	2,689	960	2,312	41.52
16	81	55	228	24.12	7.71	3,150	324	713	45.44
17	92	21	51	41.18	22.93	540	46	91.6	50.22
18	85	20	48	41.67	13.33	505	89	150	59.33

^aNumber of OTUs in Sanger sequencing divided by Sanger Chao1 (percent).

^bNumber of OTUs in Sanger sequencing divided by pyrosequencing Chao1 (percent).

^cNumber of OTUs in pyrosequencing divided by pyrosequencing Chao1 (percent).