FIG. 1.

(A) Physical map of the Tn1409 transposon vector pXX2 carrying the lux operon. Amp, ampicillin resistance; cmx, chloramphenicol resistance; tnpA, transposase IS1409; IRR, right inverted repeat; IRL, left inverted repeat; ORI, origin of replication. The arrows indicate locations of the primers used for sequencing. (B) Representative image of bioluminescent mutants recovered on an SB plate screened by IVIS. (C) PCR analysis with CmxF/CmxR and LuxCF/R primers of the colonies recovered after pXX2 electroporation. Lane 1, 1-kb-plus ladder; lanes 2 and 7, wild-type C290 as a negative control; lanes 3 and 8, pXX2 plasmid as a positive control; lanes 4 to 6 and 9 to 11, bioluminescent transposon mutants. Only results from representative strains are shown.