TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| C. michiganensis subsp. michiganensis | ||
| C290 | Wild-type virulent strain isolated in Ohio | Laboratory collection |
| A300 | Wild-type virulent strain isolated in Ohio | Laboratory collection |
| C. michiganensis subsp. nebraskensis LexIIC | Wild-type virulent strain isolated in Nebraska | D. L. Coplin |
| E. coli | ||
| DH5α | Used in cloning experiments | Invitrogen |
| ER2925 | dam and dcm methylation-negative strain | New England Biotechnology |
| Plasmids | ||
| pXen5 | Source for promoterless lux operon optimized for expression in Gram-positive bacteria; Ery Kan; 18.3 kb | Xenogen; 14 |
| pXen13 | Source for Gram-negative lux operon from P. luminescens; Amp; 8.7 kb | Xenogen |
| pMod3 | Contains EZ::TN transposon; 2.8 kb | Epicentre |
| pKGT452Cβ | Tn1409 Cβ; tnpA cmx Amp Cm; 6.4 kb | 25 |
| pUC4K | Source of kanamycin resistance marker; Kan Amp | Amersham |
| pUWGR4 | pMod3 containing EZ::TN transposon and lux operon from pXen13; Kan; 10 kb | 33 |
| pXX1 | pMod3 containing promoterless lux operon from pXen5; Kan; 9.3 kb | This study |
| pXX2 | pKGT452Cβ containing promoterless lux operon from pXen5; Amp Cm; 12.2 kb | This study |
| pXX3 | pKGT452Cβ containing promoterless lux operon from pXen13; Amp Cm; 12.3 kb | This study |
a
Kan, kanamycin resistance; Amp, ampicillin resistance; Cm, chloramphenicol resistance; Ery, erythromycin resistance.