TABLE 2.
Oligonucleotides used in this study
| Name | Sequencea | Purpose |
|---|---|---|
| LuxpXen5F | 5′ ATAAAAGAATTCGACTCCTGTGAAATGATC | Amplification of the lux operon and kanamycin gene from pXen5 |
| LuxpXen5R | 5′ AAAAAACTCGACCGGATGTACTTCAGAAAAGA | |
| pKGT1F | 5′ AAAAAAGCGGCCGCATCACGCCGCTAGAGCTT | Inverse PCR amplification of pKGT452Cβ to clone lux operon from pXen5 |
| pKGT1R | 5′ AAAAAACTCGAGTCAGAGAAGGTGAGGGCCTC | |
| KanF | 5′ GAAGCGTTTGATAGTTAAGT | For confirmation of EZ::TN<lux-kan> integration into chromosome |
| KanR | 5′ GGTACTAAAACAATTCATCC | |
| LuxpXen5F2 | 5′ ATAAAAGCGGCCGCGAAACAGCTATGACCATGAT | Amplification of the lux operon from pXen5 |
| LuxpXen5R2 | 5′ AAAAAACTCGAGTTATTATTTCCCTCCTCGAC | |
| pKGT2F | 5′ AAAAAACTCGAGATCACGCCGCTAGAGCTTGG | Inverse PCR amplification of pKGT452Cβ to clone lux operon from pXen13 |
| pKGT2R | 5′ AAAAAGCGGCCGCTCAGACAAGGTGAGGGCCTC | |
| CmxF | 5′ AGAGTACTGCCGACGCCGA | Confirmation of Tn1409-lux integration into chromosome |
| CmxR | 5′ ACTGTCGATCCTGCTCTCCG | |
| LuxCF | 5′ GTTGATGAATATCCACCTCT | Confirmation of Tn1409-lux integration into chromosome |
| LuxCR | 5′ GGACATACATTCGTGACTTA | |
| Xu3F | 5′ GGATTTGTCGGGGTGTTTCG | Mapping of insertion site of Tn1409-lux in C. michiganensis subsp. michiganensis genome |
| Xu3R | 5′ CGGCCCCACAGAAGCAATTA |
a
Restriction sites added to the 5′ end of each primer are underlined.