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. 2010 Apr 23;76(12):4008–4018. doi: 10.1128/AEM.02740-09

FIG. 5.

FIG. 5.

(A) Strategy for assembly of the caprazamycin gene cluster and the l-rhamnose subcluster. aac(3)IV, apramycin resistance gene; aad4, spectinomycin-streptomycin resistance gene; hyg, hygromycin resistance gene; loxP, Cre recognition site; oriT, origin of transfer. The size of the resistance cassettes and their components are not drawn to scale. (B) LC-ESI-MS UV chromatogram and product ion chromatograms from mass scans for caprazamycins E/F with m/z 1,118.6 [M+H]+, caprazamycins C/D/G with m/z 1,132.6 [M+H]+, and caprazamycins A/B with m/z 1,146.6 [M+H]+ in butanolic extracts of S. coelicolor M512/cpzEW07.