TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant feature(s)a | Reference or source |
|---|---|---|
| B. coagulans strains | ||
| DSM 1 | Type strain | DSMZ collection |
| DSM 1 ΔsigF | DSM 1 ΔsigF | This study |
| DSM 1 ΔlacZ | DSM 1 ΔlacZ | This study |
| DSM 1 ΔlacZ ΔsigF | DSM 1 ΔlacZ ΔsigF | This study |
| Plasmids | ||
| pNZ124 | 2.8 kb, Cmr Ts, Gram-positive-E. coli shuttle vector | 21 |
| pNZ8048 | 3.3 kb, Cmr, pNZ124-based cloning vector for the use of the NICE system | 13 |
| pNW33n | 4.2 kb, Cmr, Geobacillus-E. coli shuttle vector | Bacillus Genetic Stock Centre |
| pMH66 | 5.1 kb, Tetr Ts, pNZ124-based Cre-encoding plasmid | This study |
| pMH77 | 3.0 kb, Cmr Ts, pNZ124-based cloning vector with lox66-cat-lox71 cassette | This study |
| pMH79 | 5.0 kb, Cmr Ts, pMH77-based integration vector for sigF deletion | This study |
| pJS27 | 5.5 kb, Cmr, pNW33n derivative containing Pamy-ldhA | This study |
| pLAC | 5.1 kb, Cmr Ts, pMH77-based integration vector for lacZ deletion | This study |
| pNZlac | 5.5 kb, Cmr Ts, pNZ124-based cloning vector with B. coagulans DSM 1 lacZ gene | This study |
| pSPOIIAA-LAC | 5.7 kb, Cmr Ts, pNZ8048 containing PspoIIAA-lacZ fusion | This study |
| pSPOIID-LAC | 5.9 kb, Cmr Ts, pNZ8048 containing PspoIID-lacZ fusion | This study |
| pDACF-LAC | 5.8 kb, Cmr Ts, pNZ8048 containing PdacF-lacZ fusion | This study |
| pCOTE-LAC | 5.7 kb, Cmr Ts, pNZ8048 containing PcotE-lacZ fusion | This study |
| pPTA-LAC | 5.8 kb, Cmr Ts, pNZ8048 containing Ppta-lacZ fusion | This study |
| pLDHL-LAC | 6.7 kb, Cmr Ts, pNZ8048 containing PldhL1-lacZ fusion | This study |
| pPGI-LAC | 6.2 kb, Cmr Ts, pNZ8048 containing Ppgi-lacZ fusion | This study |
a
Cmr, chloramphenicol resistant; Tetr, tetracycline resistant; Ts, thermosensitive replicon in B. coagulans.