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. 2010 Apr 23;76(12):4092–4095. doi: 10.1128/AEM.03034-09

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FIG. 2. — Transformation efficiency of plasmids into X. fastidiosa. Efficiency of transformation was calculated as the number of transformants per microgram of DNA. The amount of DNA was quantified by measuring absorbance at 260 nm by using a NanoDrop spectrophotometer (Thermo Scientific). The means of the results for three independent experiments and their standard errors are indicated. (A) X. fastidiosa-isolated pBBR1MCS-5. (B) DH5α-isolated pBBR1MCS-5 alone. (C) DH5α-isolated pBBR1MCS-5 with TRI. (D) DH5α-isolated pAM232 with TRI. (E) DH5α-isolated pBBR1MCS-5 treated with M.SssI according to manufacturer's instructions before coelectroporation with TRI. (F) EAM1-isolated pBBR1MCS-5 with TRI.