TABLE 1.
Bacteria, plasmids, and oligonucleotides used in this study
| Strain, plasmid or oligonucleotide | Description | Reference or source |
|---|---|---|
| Strains | ||
| Xylella fastidiosa Temecula1 | X. fastidiosa subsp. fastidiosa; PD isolate ATCC 700964 | 8 |
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1 | Lab collection |
| DH5α λpir | DΗ5α lysogenized with λ pir bacteriophage | Lab collection |
| EAM1 | DH5α derivative; Spr StrattPHK022::(PLlacO-1-PD1607) | This study |
| Plasmids | ||
| pAH69 | Apr; CRIM helper plasmid; oriR101 IntHK022 | 11 |
| pAH144 | Spr Str; R6K γ ori attPHK022 plasmid dependent upon pir+ in host | 11 |
| pAM217 | Apr; PD1607 in pJET1.2/blunt | This study |
| pAM218 | Apr; PD1607 from pAM217 in pZE12 | This study |
| pAM224 | Spr Str; PLlacO-1-PD1607 from pAM218 in pAH144 | This study |
| pAM232 | Gmr; disrupted NspV site on pBBR1MCS-5 by filling-in with Klenow | This study |
| pBBR1MCS-5 | Gmr; pBBR1 replicon; broad-host-range cloning vector | 15 |
| pJET1.2/blunt | Apr; blunt PCR cloning vector | Fermentas |
| pZE12 | Apr; ColE origin; PLlacO-1 promoter | 18 |
| Oligonucleotides | ||
| PD1607-Kpn | 5′-GGTACCGTGAACGAAGCAAAGAAACG-3′ (KpnI site is underlined) | |
| PD1607-Xba | 5′-TCTAGACTATGCGGCGCGGCGCTTGTGCGG-3′ (XbaI site is underlined) |