FIG. 3.
Qualitative fluorescence HPLC analyses of wild-type A. fumigatus (wt), an easA disrupted strain (ΔeasA disruptant 8 [ΔeasA 8]), and ΔeasA disruptant 8 augmented with a functional copy of C. purpurea easA (ΔeasA easACp mutant). B, fumigaclavine B; Ch, chanoclavine-I; Ald, chanoclavine-I-aldehyde; S/I, setoclavine or isosetoclavine; F, festuclavine; A, fumigaclavine A; Ag, agroclavine; and C, fumigaclavine C. The relative sequence of elution of the diastereoisomers setoclavine and isosetoclavine has not been determined in our chromatographic system; thus, the two peaks resulting from oxidation of agroclavine and having spectral properties of setoclavine and isosetoclavine are both labeled with S/I in the figure. Excitation and emission wavelengths are provided in the upper left corner of each chromatogram.