TABLE 2.
Fibrolytic activities in the spent cultures of strain H1 growing on cellulose-rich filter paper versus xylan-rich materialsa
Enzyme | Mean sp act (mU/mg of protein) ± SD |
||
---|---|---|---|
Corn cob | Birchwood xylan | Filter paper | |
Endoglucanase | 52.4 ± 4.8 | 1,279.1 ± 45.6 | 629.9 ± 117.1 |
Cellobiohydrolase | 0.2 ± 0.1 | ND | 2.4 ± 0.1 |
β-Glucosidase | 1.4 ± 0.1 | 0.4 ± 0.4 | 44.2 ± 2.3 |
Xylanase | 567.7 ± 12.8 | 7,000.8 ± 184.4 | 2,002.0 ± 253.7 |
β-Xylosidase | 6.5 ± 0.1 | 19.5 ± 1.4 | 4.3 ± 0.3 |
Mannanase | 40.5 ± 2.9 | 509.7 ± 40.2 | 495.4 ± 6.2 |
Arabinofuranosidase | 11.0 ± 0.9 | 31.2 ± 0.6 | 91.8 ± 13.8 |
Acetyl esterase | 4.2 ± 0.6 | 17.1 ± 1.4 | 10.8 ± 1.9 |
Feruloyl esterase | 0.6 ± 0.1 | 2.7 ± 0.0 | 5.8 ± 0.1 |
Pectinase | 35.1 ± 2.5 | 20.2 ± 1.0 | 2.6 ± 1.7 |
The activity values are the averages of the values determined from three independent experiments. ND, not determined.