TABLE 5.
Enzymatic activity assays of Avicel-bound proteins by zymography and E. coli expressed proteins of strain H1
| SDS-PAGE band | Gene | ORF no.a | Enzymatic activity | Method(s) |
|---|---|---|---|---|
| 2 | Cel5A | 09989 | Endoglucanase/xylanase | Zymography |
| 3 | Cel48A | 04820 | Cellobiohydrolase | Overexpressed protein |
| 4 | Cel9A | 04825 | Endoglucanase/pectinase | Overexpressed protein/zymography |
| 5 | Xyn43A | 10615 | Xylanase | Zymography |
| 6 | Cel9B | 07788 | Xylanase | Zymography |
| Xyn10A | 10610 | Xylanase | Zymography | |
| Xyn30A | 04919 | Xylanase | Zymography | |
| 7 | Fae1A | 18248 | Feruloyl esterase | Overexpressed protein |
a
All the ORFs had a prefix of CGSCsYakCAS.