TABLE 6.
Enzymatic characterization of overexpressed Avicel-bound proteins
| ORF no.a | Enzymatic activityb | Km | Mean sp act (mU/mg) ± SD | Optimum temp (°C) | pH optimum |
|---|---|---|---|---|---|
| 04820 | Cellobiohydrolase | 0.4 mM | 0.4 ± 0.1 | 42 | 7.0 |
| 04825 | Endoglucanase | 3.8 mg/ml | 28,200 ± 2,200 | 45 | 6.0 |
| 18248 | Feruloyl esterase | 1.7 mM | 353.2 ± 6.2 | 40 | 6.8 |
a
All of the ORFs had a prefix of CGSCsYakCAS.
b
The cellobiohydrolase activity was determined on pNPC, the endoglucanase activity was determined on CMC, and the feruloyl esterase activity was determined on ferulic acid methylester.