TABLE 1.

Sequences of primers and hydrolysis probes (5′ FAM-3′ TAMRA labeled) designed to detect Anabaena and Microcystis mcyE genes in qPCR

Primer, probe, or parameter	Sequence or size for mcyE gene of:
	Anabaena	Microcystis
Primer
Forward	611F: 5′-CTAGAGTAGTCACTCACGTC-3′	127F: 5′-AAGCAAACTGCTCCCGGTATC-3′
Reverse	737R: 5′-GGTTCTTGATAGTTAGATTGAGC-3′	247R: 5′-CAATGGGAGCATAACGAGTCAA-3′
Probe	672P: 5′-CAAGTTCCCACAATTCTTGGATTAGCAGC-3′	186P: 5′-cAATGGTTATCGAATTGACCCCGGAGAAAT-3′
Amplicon length (bp)	126	120