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. Author manuscript; available in PMC: 2010 Jun 29.
Published in final edited form as: J Hepatol. 2005 Apr;42(4):548–556. doi: 10.1016/j.jhep.2004.11.043

Fig. 4.

Fig. 4

Expression of active MMP-9, TIMP-2, MT1-MMP, or uPA are not increased in IL-6−/− mice following administration of CCl4. Immunoblot of whole liver extracts prepared from IL-6+/+ and IL-6−/− livers following a single dose of CCl4 were probed with antibodies to MMP-9, TIMP-2, MT1-MMP, and uPA. (A) An increase in latent but not active MMP-9 is apparent in IL-6−/− livers. (B) Decreased TIMP-2 expression in IL-6−/− livers is apparent, concomitant with increased MMP-2. (C) A transient peak of MT1-MMP expression at 12 h precedes activation of MMP-2 then declines rapidly in IL-6−/− animals at 36 and 48 h. (D) uPA expression declines in concert with MMP-2 activation in IL-6−/− livers. β-Actin protein expression was used as a loading control on each blot. Experiments were performed in duplicate.