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. 2009 Sep 23;30(1):140–149. doi: 10.1038/jcbfm.2009.198

Figure 4.

Figure 4

Twenty-four hours after CCAo/MCAo, Long Evans rats received an intra-carotid injection of MNCs. The MNCs were derived from the bone marrow and labeled with Q tracker nanocrystals before infusion. Fluorescence microscopic images illustrate time-dependent elimination of MNCs in per-infarcted rat brain. One hour (A), 6 h (B), 24 h (C), and 7 days (D) after intra-carotid injection. Green: Fluorescein isothiocyanate (to unspecifically label the brain) and red: Q-Tracker. Magnification, × 40. (E) A histogram showing a reduction in the number of labeled MNCs in the peri-infarcted zone as a function of time (n=4 per group). (F to H) Fluorescence microscopic images of two different areas in the peri-infarct region illustrating Q-dot-labeled MNCs (left panel), TUNEL-stained cells in the same region (middle panel), and colocalization of Q-MNCs with TUNEL-positive immunoreactivity. Colocalization of Q-MNCs with TUNEL is represented by yellow fluorescence due to merging of red fluorescence with the green fluorescence (right panel). Magnification, × 40. (I) A diagram to show the borderzone area of the infarct (peri-infarct) where samples were obtained for histological analysis.