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. 2010 Jul;12(4):441–452. doi: 10.2353/jmoldx.2010.090214

Figure 2.

Figure 2

General strategy for the MLL pairing partner D-FISH assay. A: Each of the five assays uses the MLL probe labeled in green and a second probe labeled in red for one of the translocation partner genes (AFF1, MLLT4, MLLT3, ELL, or MLLT1). B: Ideograms of chromosome 4 and 11 with red (R) and green (G) ovals representing the 4q21 AFF1 and 11q23 MLL probe regions, respectively. Normal chromosome four homologues display a 2R signal pattern and normal chromosome 11 homologues display a 2G signal pattern. Reciprocal translocation of chromosomes 4 and 11 resulting in disruption of AFF1 and MLL causes splitting of one of the R and G signals, respectively. Subsequent juxtaposition of the split AFF1 and MLL signals results in two sets of paired R and G signals, observed as two yellow F signals. The normal chromosomes 4 and 11 remain as separate R and G signals. An overall 1R1G2F FISH signal pattern is observed.