Table 1.
Conventional Cytogenetic and D-FISH Results Using a Homebrew MLL/AFF1 Probe for 25 Patients with t (4;11) (q21;q23)
| Conventional cytogenetic analysis |
FISH analysis* |
|||
|---|---|---|---|---|
| Patient no. | Age (years) | Karyotype | Abnormal nuclei (%) | Interphase signal pattern |
| 1 | 4 M | 46,XY,t (4;11) (q21;q23) [15]/46,XY[5] | 95 | 1R1G2F |
| 2 | 6 M | 46,XY,t (4;11) (q21;q23) [30] | 92.2 | 1R1G2F |
| 3 | 21 M | 49-53,XX,+X,+add(1) (p13),t (4;11) (q21;q23),+der(4)t (4;11) (q21;q23),+5,del(6) (q21q25),+8,+8,+13[cp20] | 91.6 | 1R1G3F |
| 4 | 4 | 46,XX,t (4;11) (q21;q23) [4]/46,XX[16] | 85.2 | 1R1G2F |
| 5 | 17 | 46,XY,t (4;11) (q21;q23) [3]/46,XY[3] | 88.8 | 1R1G2F |
| 6 | 18 | 46,XY,t (4;11) (q21;q23) [3]/46,XY[3] | 92.4 | 1R1G2F |
| 7 | 24 | 48,XX,+X,add(1) (p36.3),+4,add(4) (q12),t (4;11) (q21;q23) [cp4]/ 48,idem,t (1;19) (q23;p13.3) [1] | 95.8 | 1R1G3F |
| 8 | 30 | 47,XX,+X,t (4;11) (q21;q23) [cp10]/48,idem,+8,i(17) (q10) [3]/46,XX,t (4;11) (q21;q23),add(21) (q22) [7] | 98.6 | 1R1G2F |
| 9 | 31 | 46,XY,t (4;11) (q21;q23) [20] | 97.6 | 1R1G2F |
| 10 | 31 | 48,XX,t (4;11) (q21;q23),+6,+13[9]/46,XX[11] | 95.6 | 1R1G2F |
| 11 | 38 | 46,XX,t (4;11) (q21;q23),i(7) (q10) [9]/46,XX[11] | 92.4 | 1R1G2F |
| 12 | 39 | 46,XY,t (4;11) (q21;q23) [4]/46,XY[8] | 94.8 | 1R1G2F |
| 13 | 40 | 46,XY,t (4;11) (q21;q23) [20] | 97 | 2R2G1F |
| 14 | 41 | 46,XY,t (4;11) (q21;q23) [5]/46,XY[8] | 94.2 | 1R1G2F |
| 15 | 42 | 46,XX,t (4;11) (q21;q23) [19]/46,XX[1] | 98.2 | 1R1G2F |
| 16 | 45 | 46,XX,t (4;11) (q21;q23) [20] | 91 | 1R1G2F |
| 17 | 51 | 46,XX,t (4;11) (q21;q23),add(12) (p11.2) [11]/46,XX[9] | 97.8 | 1R1G2F |
| 18 | 54 | 46,XY,t (4;11) (q21;q23) [14] | 95.4 | 1R1G2F |
| 19 | 58 | 46,XX,t (4;11) (q21;q23) [14]/46,XX[6] | 94.4 | 1R1G2F |
| 20 | 58 | 47,XX,+X,t (4;11) (q21;q23) [14]/46,XX[6] | 85 | 1R1G2F |
| 21 | 58 | 46,XX,t (4;11) (q21;q23) [6]/46,XX[14] | 50.4 | 1R1G2F |
| 22 | 65 | 46,XX,t (4;11) (q21;q23) [6]/46,XX[3] | 96.2 | 1R1G2F |
| 23 | 65 | 46,XX,t (4;11) (q21;q23) [15]/47,idem,+6[1]/46,XX[4] | 94.4 | 1R1G2F |
| 24 | 74 | 47,XX,+X,dup(1) (q21q25),add(2) (q11.2),add(4) (q35),del(9) (q13q22), der(11)t (4;11) (q21;q23),−18,+19[cp4]/46,XX[16] | 0 | 2R2G |
| 25 | 80 | 41-43,XX,add(1) (q21),t (4;11) (q21;q23),−5,del(9) (p13),−10,i(14) (q10),−15,del(16) (q12.1),−17,−19,−20,+4mar[cp18]/46,XX[3] | 27.6 | 3R1G |
F, fusion (yellow); G, green; M, months; R, red.
*
A total of 500 interphase nuclei were analyzed by two technologists (250 nuclei each).