Table 2.
Conventional Cytogenetic and D-FISH Results Using a Homebrew MLL/MLLT4 Probe for 20 Patients with t (6;11) (q27;q23)
| Patient no. | Age (years) | Conventional cytogenetic analysis Karyotype | FISH analysis* Abnormal nuclei (%) | Interphase signal pattern(s) |
|---|---|---|---|---|
| 26 | 5 M | 50,XX,+5,t (6;11) (q27;q23),+12,+15,+18[8]/51-52,idem,+4,+15[cp9]/46,XX[3] | 35.8 | 1R1G2F |
| 27 | 5 | 45,XX,t (6;11) (q27;q23),+der(6)t (6;11),−9,−20[1]/47,XX, t (6;11) (q27;q23),+der(6)t (6;11),add(10) (p15) [19] | 88.4 | 1R1G3F |
| 28 | 10 | 46,XY,der(11)del(11) (p13)add(11) (q21),add(12) (p11.2) [5]/46,sl, t (8;18)(q13;q21) [15].ish t (6;11) (q27;q23) (3′MLL+;5′MLL+) | 11.6, 85.2 | 1R2G1F, 1R3G1F |
| 29 | 11 | 46,XX,t (6;11) (q27;q23),t (15;19) (q24;p13.3)?c[14]/92,XXXX, add(1) (p32),add(5) (q13),t (6;11) (q27;q23)x2, t (15;19) (q24;p13.3)?cx2[6] | 80, 13.6 | 1R1G2F, 2R2G4F |
| 30 | 25 | 47,XY,der(6)t (6;11) (q27;q23),der(11)add(11) (p11.2)t (6;11),+21[19]//46,XX[1] | 74.8 | 1R1G2F |
| 31 | 26 | 46,XX,t (6;11) (q27;q23) [20] | 98 | 1R1G2F |
| 32 | 29 | 46,XY,t (6;11) (q27;q23) [10]/47,XY,idem,+9[10] | 96.6 | 1R1G2F |
| 33 | 33 | 46,XY,t (6;11) (q27;q23) [20] | 87.6 | 1R1G2F |
| 34 | 38 | 46,XX,t (6;11) (q27;q23) [20] | 97.6 | 1R1G2F |
| 35 | 38 | 46,XY,t (6;11) (q27;q23) [2]/46,XY[18] | 6.8 | 1R1G2F |
| 36 | 41 | 46,XY,t (6;11) (q27;q23) [15]/54,XY,+4,t (6;11)(q27;q23),+der(6)t (6;11),+8,+8,+12,+15,+16,+18[8] | 4.6, 6.6, 7, 65.6 | 1R1G3F, 2R1G2F, 2R1G1F, 1R1G2F |
| 37 | 42 | 46,XX,t (6;11) (q27;q23) [cp10]/47,idem,add(4) (q31.1),+8[cp3]/51,idem,+X,+6,+8,+15,+21[3]/46,XX[4] | 15.8, 65.6 | 2R1G2F, 1R1G2F |
| 38 | 44 | 46,XX,t (6;11) (q27;q23) [13]/48,sl,+19,+21[2]/46,XX[5] | 11.4 | 1R1G2F |
| 39 | 55 | 46,XX,t (6;11) (q27;q23) [1]/50,idem,+19,+21,+21[18]/46,XX[1] | 1.2, 5.8, 44.2 | 2R1G1F, 1R1G2F, 2R1G2F |
| 40 | 55 | 46,XY,t (6;11) (q27;q23) [20] | 88 | 1R1G2F |
| 41 | 58 | 46,XX,t (6;11) (q27;q23) [14]/46,XX[6] | 94 | 1R1G2F |
| 42 | 60 | 46,XY,t (1;5) (p22;q11.2)?c,t (6;11) (q27;q23) [20] | 95.8 | 1R1G2F |
| 43 | 65 | 47,XY,t (6;11) (q27;q23),+8[20] | 95.6 | 1R1G2F |
| 44 | 81 | 45,X,−Y,t (6;11) (q27;q23) [20] | 98.4 | 1R1G2F |
| 45 | 86 | 47,XX,t (6;11) (q27;q23),+19[8]/46,XX,del(11) (q13q23) [4]/46,XX[8] | 0 | 2R2G |
F, fusion (yellow); G, green; M, months; R, red.
*
A total of 500 interphase nuclei were analyzed by two technologists (250 nuclei each).