Table 5.

Conventional Cytogenetic and D-FISH Results Using a Homebrew MLL/MLLT1 Probe for 20 Patients with t (11;19) (q23;p13.3)

		Conventional cytogenetic analysis	FISH analysis*
Patient no.	Age (years)	Karyotype	Abnormal nuclei (%)	Interphase signal pattern(s)
84	4 W	46,XY,t (11;19) (q23;p13.3) [7]/46,XY[13]	92	1R2G1F
85	12 M	46,XY,t (11;19) (q23;p13.3) [3]/46,XY,del(6) (q21q23),t (11;19) (q23;p13.3) [7]/46,XY[10]	91	2R2G1F
86	22 M	46,XY,t (11;19) (q23;p13.3) [2]/46,XX[18]	52.2	1R1G2F
87	12	46,XX,t (9;17) (q34;q25),t (11;19) (q23;p13.3) [17]/46,XX[3]	72.4	1R1G2F
88	15	46,XX,t (11;19) (q23;p13.3) [30]	87.4	1R1G2F
89	16	52,XX,+X,+5,+6,+8,t (11;19) (q23;p13.3),+19,+21[19]/46,XX[1]	5.8, 52.4	1R1G2F, 2R1G2F
90	20	46,XX,t (11;19) (q23;p13.3) [15]/46,XX[5]	90.8	1R1G2F
91	20	46,XX,t (11;19) (q23;p13.3) [13]/46,XX[7]	91	1R1G2F
92†	20	45-47,XY,i(9) (q10),t (11;19) (q23;p13.3),+2-3mar[cp3]/46,XY[27]	0.4	1R1G2F
93	23	48,XY,+8,t (11;19) (q23;p13.3),+20[2]/48,idem,del(5) (q22q33) [16]/ 48,idem,t (2;12;10) (q37;q13;q22) [2]	89.6	1R1G2F
94	30	46,XX,t (11;19) (q23;p13.3) [16]/46,XX[4]	86.2	1R1G2F
95	36	47,XXY?c,t (11;19) (q23;p13.3) [20]	98.6	1R1G2F
96‡	37	46,XX,t (11;19) (q23;p13.3) [20]	75	1R1G2F
97‡	39	46,XY,t (11;19) (q23;p13.3),der(20)t (17;20) (q11.2;q13.3) [20]	96.6	1R1G2F
98	43	46,XY,t (11;19) (q23;p13.3) [18]/47,idem,+8[2]	1.6, 92.4	1R1G3F, 1R1G2F
99	45	47,XX,+X,t (11;19) (q23;p13.3) [19]/46,XX[1]	95	1R1G2F
100	47	46,XY,t (11;19) (q23;p13.3) [20]	96.4	1R1G2F
101	53	46,XX,t (11;19) (q23;p13.3) [9]/46,XX[1]	94.8	1R1G2F
102	71	47,XX,+X,t (11;19) (q23;p13.3) [4]/46,XX[26]	92.2	1R1G2F
103‡	75	46,XX,t (11;19) (q23;p13.3) [20]	96.4	1R1G2F

F, fusion (yellow); G, green; M, months; R, red; W, weeks.

^*A total of 500 interphase nuclei were analyzed by two technologists (250 nuclei each).

^†This sample represents a posttherapy sample.

^‡The breakpoint for these patients was initially evaluated as 19p13.1.