Table 2.
Probe Primer Sequences Used for Multiplex Detection of 11 Blood Group SNPs
| System | Allele | Polymorphism | Position change | Location | Forward/ reverse | Extension primer | Size (b) | Conc. (μM) |
|---|---|---|---|---|---|---|---|---|
| FY | FY*1/FY*2 | Gly42Asp | 125G>A | Exon-2 | Forward | 5′-TGAATGATTCCTTCCCAGATGGAGACTATG-3′ | 30 | 0.04 |
| FY | C-wt/FY*X | Arg89Cys | 265C>T | Exon-2 | Forward | 5′-13T-GCTTTTCAGACCTCTCTTC-3′ | 32 | 1.20 |
| DO | DO*1/DO*2 | Asn265Asp | 793A>G | Exon-2 | Forward | 5′-TTGTTTAAAGTTATAAATATGAGCTACCACCCAAGAGGA-3′ | 39 | 0.25 |
| MNS | MNS*3/MNS*4 | Met29Thr | 143T>C | Exon-4 | Forward | 5′-cttatttggacttacattgaaattttgctttatagGAGAAA-3′ | 41 | 2.30 |
| JK | JK*1/JK*2 | Asp280Asn | 838G>A | Exon-9 | Reverse | 5′-16T-TGTTGAAACCCCAGAGTCCAAAGTAGATGT-3′ | 46 | 0.35 |
| CO | CO*1/CO*2 | Ala45Val | 134C>T | Exon-1 | Forward | 5′-20T-TTCAAATACCCGGTGGGGAACAACCAGACGG-3′ | 51 | 0.28 |
| MNS | g-wt/GYPBS-Int5 | IVS5, +5 | +5g>t | Intron-5 | Forward | 5′-24T-TTACASTATTCGCCGACTGATAAAGgtga-3′ | 53 | 0.016 |
| KEL | KEL*1/KEL*2 | Met193Thr | 698T>C | Exon-6 | Forward | 5′-24T-TGGTAAATGGACTTCCTTAAACTTTAACCGAA-3′ | 56 | 0.3 |
| YT | YT*1/YT*2 | His353Asn | 1057C>A | Exon-2 | Forward | 5′-40T-CTCATCAACGCGGGAGACTTC-3′ | 61 | 1.60 |
| MNS | C-wt/GYPBS−230 | Thr58Met | 230C>T | Exon-5 | Forward | 5′-43T-TGATGGCTGGTATTATTGGAA-3′ | 64 | 2.30 |
| FY | FY*wt/FY*Fy | Gata wt/mt | -33T>C | Promoter | Reverse | 5′-48T-AGCGCCTGTGCTTCCAAG-3′ | 66 | 0.02 |
Lower case letters refer to intron sequences. Bold characters refer to the 5′ poly-thymidine tail.