Table 2.

Thermocycling Parameters and Instrumentation for the PCR Methods

Method	Instrumentation	PCR conditions
HybProbe real-time PCR29	Roche LightCycler version 1.5	95°C/10 minutes; 40 cycles of 95°C/10 seconds; 50°C/20 seconds; 72°C/30 seconds
TaqMan real-time PCR	ABI Prism 7000 SDS	95°C/10 minutes; 40 cycles of 95°C/15 seconds; 60°C/1 minute; hold at 25°C/1 minute
SYBR Green real-time PCR	Stratagene MX4000	95°C/10 minutes; 40 cycles of 95°C/30 seconds; 55°C/1 minute; 72°C/30 seconds
LUX real-time PCR28	Cepheid SmartCycler	95°C/3 minutes; 40 cycles of 95°C/10 seconds; 55°C/15 seconds; 72°C/15 seconds
Conventional PCR22	ABI GeneAmp 9600	95°C for 5 minutes; 40 cycles of 94°C/40 seconds; 56°C/40 seconds and 72°C/40 seconds; final extension at 72°C/6 minutes