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. 2010 Jul;12(4):520–524. doi: 10.2353/jmoldx.2010.090218

Figure 1.

Figure 1

Detection of a novel intronic insertion within an e14/a2 BCR-ABL rearrangement. A: Using multiplex RT-PCR, an atypical BCR-ABL transcript was observed in the patient sample (p) as compared with e14/a2 (c1) and e1/a2 (c2) controls. Lane m: 100-bp ladder. B: Direct sequencing of the RT-PCR product revealed a 42-nucleotide insertion of ABL intron Ib within an e14/a2 BCR-ABL rearrangement. The resulting in-frame insertion of 15 amino acids was shown. C: A well-conserved donor splicing site was detected, at the genomic DNA level, in the 3′ end of the insertion. Uppercase and lowercase nucleotides corresponded respectively to pseudo-exonic and intronic sequences.