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Identification of the BCR-ABL breakpoint on genomic DNA. A: As we hypothesized that the BCR-ABL breakpoint occurred near the 5′ end of the inserted sequence, five primers located in BCR introns 13 and 14 and a reverse primer in ABL intron Ib (at the 3′ end of the insertion) were designed. B: Specific PCR amplifications were observed with two pairs of primers (d-z and e-z).
