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. 2010 Jul;177(1):21–28. doi: 10.2353/ajpath.2010.090999

Figure 1.

Figure 1

A: Location of CpG islands in α-SMA gene. DNA sequence of α-SMA gene −2900 to + 3100 from transcriptional start site was submitted to the EMBOSS CpGPlot/CpGReport/Isochore program online. The results revealed three islands between bp 2142 to 2244 (corresponding to −759 to −656 from transcriptional start site, namely promoter region), bp 3095 to 3199 (corresponding to + 195 to + 299 from transcriptional start site, namely first intro region1), and bp 3339 to 3452 (corresponding to + 439 to + 552 from transcriptional start site, namely first intron region 2). B: Methylation status of CpG islands in the α-SMA gene. This was determined by DNA pyrosequencing of the α-SMA gene promoter and the first intron regions 1 and 2 where CpG islands were located. The position of each methylated cytidine from the transcriptional start site was indicated in the x axis. The results were expressed as percentages and shown as the means ± SE from three independent experiments.