Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cyclic stretch induced calcification of porcine aortic valve cusps. Aortic valve cusps were exposed to 10% (A and B) or 15% (C and D) cyclic stretch for seven days in fully osteogenic medium containing both high phosphate and TGF-β1. Cusps were then stained with Alizarin Red (A and C) or Von Kossa (B and D). Calcific nodules are marked with arrows. F indicates fibrosa; V, ventricularis.
