Table 1.
Different Formulations of Culture Media Used in Order to Develop a Model for Calcification in an ex vivo System
| No. | Culture media | Composition |
|---|---|---|
| 1 | Normal medium | Normal DMEM supplemented with 10% fetal bovine serum |
| 2 | Base osteogenic medium | Normal medium supplemented with 1 mmol/L β-glycerophosphate, 10 μmol/L dexamethasone, and 0.9 mmol/L PO43− |
| 3 | Base osteogenic medium with high phosphates | Base osteogenic medium with 3.8 mmol/L PO43− |
| 4 | Base osteogenic medium with TGF-β1 | Base osteogenic medium with 1 ng/ml TGF-β1 |
| 5 | Full osteogenic medium | Base osteogenic medium with 3.8 mmol/L PO43− and 1 ng/ml TGF-β1 |