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. 2010 Jul;177(1):58–69. doi: 10.2353/ajpath.2010.091008

Figure 6.

Figure 6

Expression of PDGFR-α on human glomerular endothelial cells in vitro and their proliferative response to PDGF-C and PDGF-C-conditioned media. PDGFR-α mRNA and protein expression were detected in CiGEnC by reverse transcription PCR (A) and immunofluorescence (B). PDGFR-α transcript expression increased with time of CiGEnC differentiation and reached its peak on day 5. PDGF-C alone did not induce CiGEnC proliferation under permissive (D) or nonpermissive temperatures (C). When added to full medium, PDGF-C induced marked proliferation of differentiated CiGEnC (C). This effect was not observed in undifferentiated cells (D). Conditioned medium from mesangial cells or macrophages stimulated with 5 ng/ml PDGF-C induced proliferation of CiGEnC at the permissive and the nonpermissive temperature (E–H). *P < 0.05 vs. unstimulated cells, **P < 0.05 vs. full medium.