Table 1.
List of Primers for Quantitative Real-Time RT-PCR Experiments
| Gene symbol accession | Primer designation | Primer sequence | Product length, bp |
|---|---|---|---|
| Col4a1 NM_009931.1 | Col4a1S1 forward | 5′-CTGGCACAAAAGGGACGAG-3′ | 238 |
| Col4a1S1 reverse | 5′-ACGTGGCCGAGAATTTCACC-3′ | ||
| Col4a2 NM_009932.2 | Col4a2S2 forward | 5′-TGCTACCCGGAGAAAGGAG-3′ | 106 |
| Col4a2S2 reverse | 5′-CTTTGCGGCCCTGTAGTCC-3′ | ||
| Col4a3 NM_007734.1 | Col4a3S7 forward | 5′-GGGACATGTAACTACTACTCAAACTCC-3′ | 88 |
| Col4a3S7 reverse | 5′-TCACAGTTGATGGAATAGGTTTTCT-3′ | ||
| Col4a4 NM_007735.1 | Col4a4S4 forward | 5′-CTGGCTTGAAGGGAGACCT-3′ | 69 |
| Col4a4S4 reverse | 5′-CTCCTGCATCACCAGGAAGT-3′ | ||
| Col4a5 NM_007736.2 | Col4a5 forward | 5′-GGAGAACGGGGGTTTCCAG-3′ | 247 |
| Col4a5 reverse | 5′-CTCCCTTGGTTCCATTGCATC-3′ | ||
| Cxcl12 NM_013655.3 | Cxcl12 forward | 5′-CAAGGTCGTCGCCGTGCTG-3′ | 122 |
| Cxcl12 reverse | 5′-CGTTGGCTCTGGCGATGTGG-3′ | ||
| Cxcr4 NM_009911 | Cxcr4 forward | 5′-CAGAGGCCAAGGAAACTGCT-3′ | 101 |
| Cxcr4 reverse | 5′-CTGACGTCGGCAAAGATGAA-3′ | ||
| Cyclophilin NM_008907 | cyclophilin forward | 5′-CAGACGCCACTGTCGCTTT-3′ | 132 |
| cyclophilin reverse | 5′-TGTCTTTGGAACTTTGTCTGCAA-3′ | ||
| Egln3 NM_NM_028133.1 | Egln3 forward | 5′-TGTCTGGTACTTCGATGCTGA-3′ | 85 |
| Egln3 reverse | 5′-AGCAAGAGCAGATTCAGTTTTTC-3′ | ||
| Epas1 NM_001430 | PPM03309A-200 | Referenceposition457–477 | 164 |
| Hif1a NM_001530 | PPM03799B-200 | Referenceposition700–720 | 160 |
| Hif3a NM_152794 | PPM05268B-200 | Referenceposition1998–2018 | 180 |
| Lama1 NM_008480.2 | Lama1P1 forward | 5′-TGTAGATGGCAAGGTCTTATTTCA-3′ | 261 |
| Lama1P1 reverse | 5′-CTCAGGCAGTTCTGTTTGATGT-3′ | ||
| Lama5 NM_001081171.1 | Lama5P2 forward | 5′-ACCCAAGGACCCACCTGTAG-3′ | 169 |
| Lama5P2 reverse | 5′-TCATGTGTGCGTAGCCTCTC-3′ | ||
| Lamb1-1 NM_008482.2 | Lamb1-1P66 forward | 5′-GGCAAACTGCAAAGTCTCG-3′ | 61 |
| Lamb1-1P66 reverse | 5′-CTGGAGGTGTTCCACAGGTC-3′ | ||
| Lamb2 NM_008483.2 | Lamb2S2 forward | 5′-GTGTGGCTTGCATAGCCCT-3′ | 122 |
| Lamb2S2 reverse | 5′-TCCGATGACTATTTGGGTTGTCT-3′ | ||
| Ngb NM_022414 | PPM28801A-200 | Referenceposition759–781 | 163 |
| Vegf NM_03376 | PPM03041E-200 | Referenceposition2467–2487 | 191 |
| Vhlh NM_000551 | PPM27529A-200 | Referenceposition2474–2493 | 190 |
Cxcr4 and Cxcl1233 and cyclophilin56 primers were designed based on prior published work. All other primers were designed by using Primer Bank (http://pga.mgh.harvard.edu/primerbank/; last accessed May 3, 2010) or the Roche Universal Probe Library (Roche Applied Science, Indianapolis, IN). Epas1, Hif1α, Hif3α, Ngb, Vegf, and Vhlh primers were purchased from SABiosciences (Frederick, MD) and sequences are unavailable; shown are the reference position obtained from SABiosciences for the amplicon. All amplified products resulted in the appropriate size band on agarose gels.