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. 2010 Jul;177(1):153–165. doi: 10.2353/ajpath.2010.090895

Figure 5.

Figure 5

Progression of liver fibrosis in HFD II-fed rabbits. Rabbits were fed SD (A and C) or HFD II (B and D) for nine months. A and B: Sirius red staining. Note collagen deposits around the portal and central vein areas, as well as bridging fibrosis in HFD II-fed rabbits. P, portal vein; C, central vein. C and D: Immunohistochemistry for αSMA, a marker of activated stellate cells and myofibroblasts. Note that αSMA+ cells are mainly seen inside fibrotic septa in the livers of HFD II-fed rabbits. Scale bar = 100 μm. E: Immunoblotting for αSMA. Note that expression of αSMA was augmented in HFD II-fed rabbits. GAPDH was the internal control. F: Expression of fibrosis-related genes in the livers of SD-fed rabbits (white bars) and HFD II-fed rabbits (gray bars) analyzed by quantitative real-time PCR. *P < 0.05; **P < 0.01.