Figure 1.

CFTR₁₇₂ synergizes with zymosan to produce neutrophil recruitment into the peritoneum. C57/B6 mice were pre-treated i.p. with various doses of CFTR₁₇₂ given either singularly at −1 hour, or twice at both −24 and −1 hour, as indicated. At time 0, mice were treated with (A) 1 mg, (B) 0.2 mg, or (C) 0.1 mg of zymosan given i.p. in 0.5 ml PBS, with lavage fluids being collected four hours later. Recruited neutrophils were stained with the anti-Gr1 Ab for flow cytometry analysis. D: Various doses of CFTR₁₇₂ alone were injected i.p. 24 hours and one hour before peritoneal lavage harvesting, and cells processed as above. Data are mean ± SEM of five mice per group. A–C: *P < 0.05, **P < 0.01 vs. zymosan/vehicle group; D: *P < 0.05 vs. naïve group.