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. 2010 Jul;177(1):208–218. doi: 10.2353/ajpath.2010.090952

Figure 5.

Figure 5

TGF-β1 disrupts cell layer integrity and paracellular permeability in primary cultured human gingival epithelial cells. A: Cells were cultured on Transwell membranes. TEER was monitored for 10 days. After reaching stable resistance on day five, TGF-β1 (5 ng/ml) or vehicle treatment was begun (arrow). TEER was recorded every day for five days after the start of treatment. Values are means ± SE of triplicate determinations; *P < 0.001 compared with vehicle treatment; †P < 0.001 compared with day three and day five; ‡P < 0.001 compared with vehicle treatment day six through day ten; §P < 0.05 compared with TGF-β1 treatment day six and day seven by analysis of variance with Bonferroni correction for multiple tests. B: Cells cultured on Transwell membranes were treated with TGF-β1 (5 ng/ml) or vehicle for five days. On day five, FITC-labeled dextran was added to the cells for one hour as described in Materials and Methods. Values are means ± SE of triplicate determinations; *P < 0.001 compared with vehicle treatment by analysis of variance.