Figure 6.

TGF-β1 inhibits cell surface E-cadherin expression in primary cultured human gingival epithelial cells. Gingival epithelial cells from a healthy donor were cultured until 80% confluent followed by treatment with TGF-β1 (5 ng/ml) or vehicle for five days. Cells were stained for E-cadherin and FITC-labeled secondary antibody for immunofluorescence on day five (right) and with DAPI to stain nuclei (left). The same fields are shown for each chromophore. Data show representative images from multiple stainings of cells at ×400 magnification; Scale bar = 0.02 mm.