Figure 3.
Global oligemia led to a reduction in total tau levels. A, B: Western blot and quantitative analysis of total tau (HT7) and phosphorylated tau (labels indicate phosphorylation site recognized by the antibody) 48-hours after insult revealed a significant decrease in total tau, tau phosphorylated at S199,202 and T181 and GSK3ß phosphorylated at Ser9 protein levels. C, D: Analysis of total and phosphotau epitopes in the formic acid fraction by Western blot analysis revealed no alterations between sham and oligemic tissue. E: Immunohistochemical analysis of sham versus oligemic mice showed a prominent reduction of total tau in the CA1, but not in the amygdala. HC (hippocampus; Scale bar = 500 μm); CA1 subfield of the hippocampus (CA1; Scale bar = 62.5 μm) and amygdala (Scale bar = 125 μm). Arrows indicate areas of decreased staining. Western blots were normalized to actin and represented as a percentage of sham.