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. 2010 Jul;177(1):311–324. doi: 10.2353/ajpath.2010.090915

Figure 4.

Figure 4

Abnormal tissue vacuolation in older GRN−/− mice. H&E staining identified vacuolation in the habenular nucleus (A and B) and the neuropil surrounding CA2-3 regions (A and C) of the hippocampus in older GRN−/− mice. The former was largely characterized by single vacuoles whereas the latter contained mainly clustered vacuoles (inset). In GRN−/− mice, habenular vacuolation was not present at 1 month (D) but was a consistent feature in older GRN−/− (E: 7 month; B: 12 month; F: 23 month] mice and absent in all GRN+/+ (G) and GRN+/− mice (H), even at 23 months. I: Clustered vacuoles were counted in CA2-3 of the hippocampus, occurring in the majority of GRN−/− mice at seven months and becoming more frequent in 12- and 23-month-old mice. These vacuoles were largely absent in GRN+/+ and GRN+/−, but if present had a low frequency. Differences in the number of clustered vacuoles between GRN−/− mice and GRN+/+ or GRN+/− mice were statistically significant for 7-, 12-, and 23-month-old mice (P < 0.05). Horizontal black bars, mean. Scale bar: 400 μm A; 30 -μm D–H.